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KMID : 1101320110430040194
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Korean Journal of Clinical Laboratory Science
2011 Volume.43 No. 4 p.194 ~ p.204
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Comparison of Histopathological Changes on the Three Drugs of Carbon Tetrachloride, Dimethylnitrosamine, Thioacetamide, and Bile Duct Ligation used for Induction of Liver Fibrosis in Rat
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Kim Jung-Hun
Park Mi-Jung
Kim Yo-El
Kim Jin-Yeong
Sin Jin-Hee
Park Su-Young
Jekal Seung-Joo
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Abstract
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This study was carried out to compare the histopathological differences of liver lesions in carbon tetrachloride (CCI4), dimethylnitrosamine (DMN), thioacetamide (TAA) and bile duct ligation (BDL)-induced rats. CCl4, DMN and TAA were administered intraperitoneally and conducted bile duct ligation for 4 weeks to induce hepatic fibrosis. Indices of liver cell injury (steatosis, hydropic degeneration, bile duct hyperplasia, hemorrhage & hemosiderin deposition), the extent of liver fibrosis (fibrotic area) and the rate of regeneration (number of PCNA-positive cells) were investigated in each group. Liver tissues were stained with hematoxylin-eosin (HE), sirius red, prussian blue and immunostained with α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), proliferative cell nuclear antigen (PCNA), and quantified using a computerized image analysis system. Liver cell steatosis was significantly increased in CCl4 and TAA groups, and hydropic degeneration and bile duct hyperplasia were significantly increased in TAA and BDL groups when compared with that in normal control, respectively. Fibrosis area was significantly increased in all four groups, especially in CCl4 group. Correlation between α-SMA and TGF-β1 expressions in four groups was good. Hemorrhage area in liver parenchyma was significantly increased in DMN group only when compared with that in normal control, while hemosiderin deposition area was significantly increased in TAA and BDL groups as well as DMN group. The Number of PCNA-positive cells was significantly increased in all four groups, especially in TAA group. These results indicate that the duration and methods of hepatotoxic drug treatment are very important factors to make plans for animal experimentation on the induction of hepatic fibrogenesis in rats.
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KEYWORD
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Hepatic fibrosis, Rat, CCl4, DMN, TAA, BDL
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